Estudio cromosómico en leucemias linfoblásticas agudas
Resumen
Se han identificado anormalidades clonales en aproximadamente 65-90% de los ninos con leucemia linfoblastica aguda (LLA). El objetivo de este estudio es identificar las alteraciones que permitan caracterizar LLA en nuestra poblacion. Se aplicaron las tecnicas citogeneticas y moleculares estandar para analizar las
celulas en medula osea. Pseudodiploidias fueron observadas en 43.4% de los casos, hiperdiploidfas en 32.9% e hipodiploidfas en 21%. Los cromosomas #3, #8, #10, #12, #15, #16, #17, #18, #19, #21, #22 y X estuvieron mayormente involucrados en diferentes tipos de cambios. El rearreglo BCR-ABL fue encontrado
en el 42.8% de los casos. Algunos de estos resultados son similares a los publicados por otros autores, por lo que pueden considerarse como evidencia de que estos cromosomas estan involucrados en rearreglos no al azar en la LLA. La determination de estas anormalidades es una herramienta util que ayudara a caracterizar la LLA, a disenar un tratamiento adecuado y ayudara en el pronostico del paciente.
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Ph 1- patients. Br J Haematol 36:347-358; 1977
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40. Barletta C, Pelicci P-G, Kenyon LC, Smith SD, Dalla- Favera R: Relationship between c-myb locus and the 6q- chromosomal aberration in leukemias and lymphomas. Science 235:1064-1067;1987
2. Rowley JD: Recurring chromosome abnormalities in leukemia and lymphoma. Semin Hematol 27:122-136:1990
3. Nowell PC: Chromosomal approaches to hematopoietic oncogenesis. Stem Cells 11:9-19;1993
4. Yunis JJ: Molecular mechanisms of hematologic malignancies. Crit Rev Oncogen 4:161-190; 1993
5. Cline MJ: The molecular basis of leukemia. N Engl J Med 330:328-336;1994
6. Farreras P, Rozman R: Hematologia. En: Medicina Interna. Edicion en CD-ROM.
7. Burmeister T, Thiel E: Molecular genetics in acute and chronic leukemias. J Cancer Res Clin Oncol 127:80- 90;2001.
8. Alter D, Mark HF: Cytogenetic study of a patient with infant acute lymphoblastic leukemia using GTG banding and chromosome painting. Exp Mol Pathol 69:152- 158:2000
9. Secker-Walker LM: Prognostic and Biological Importance of Chromosome Findings in Acute Lymphoblastic Leukemia. Cancer Genet Cytogenet 49:1-13:1990
10. Heim S, Mitelman F: Proliferation-specific and differentiation- associated chromosomal breakpoints in human neoplasia-a unifying model. Hereditas 104:307- 312:1986
11. Rowley JD: Biological implications of consistent chromosome rearrangements in leukemia and lymphoma. Cancer Res 44:3159-3168:1984
12. Secker-Walker LM, Lawler SD, Hardisty RM: Prognostic implications of chromosomal findings in acute lymphoblastic leukaemia at diagnosis. Br Med J 2:1529- 1530:1978.
13. Secker-Walker LM, Swansbury GJ, Hardisty RM, et al: Cytogenetics of acute lymphoblastic leukaemia as a factor in the prediction of long-term survival. Br J Haematol 52:389-399:1982
14. Williams DL, Harber J, Murphy SB, et al: Chromosomal translocations play a unique role in influencing prognosis in childhood acute lymphoblastic leukemia. Blood 68:205-212:1986
15. Bloomfield CD, Goldman Al, Alimena G, et al: Chromosomal abnormalities identify high-risk patients with acute lymphoblastic leukemia. Blood 67:415-420:1986
16. Pui Ch, Williams DL, Raimondi SC, et al: Hypodiploidy is associated with a poor prognosis in childhood acute lymphoblastic leukemia. Blood 70:247-253:1987
17. Williams Dl, Tsiatis A, Brodeur GM, et al: Prognostic importance of chromosome number in 136 untreated Children with acute lymphoblastic leukemia. Blood 60:864-871:1982
18. Paz-y-Mino C, Fiallo BF, Davalos V, et al: Sindromes de Fragilidad Cromosomica en el Ecuador. MetroCiencia 9:19-22:2000
19. Chomczynski P, Sacchi N: Single-Step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159:1987
20. ISCN: An International System for Human Cytogenetic Nomenclature. Report of the Standing Committe on Human Cytogenetic Nomenclature. Published in collaboration with Cytogenetics and Cell Genetics. VI. Basel: KARGER, 1995
21. Paz-y-Mino C, Burgos R, Morillo S, et al: BCR-ABL rearrangement frequencies in CML and ALL in Ecuador, South America. Cancer Genetics and Cytogenetics 1-3;2001
22. Heim S, Mitelman F: Cancer Cytogenetics. New York, Alan R. Liss. 1987
23. Sandberg AA: The Ph chromosome. En: The chromosomes in human cancer and leukemia, New York, Elsevier. 1990:331-334
24. Secker-Walker LM: The prognostic implications of chromosomal findings in acute lymphoblastic leukemia. Cancer Genet Cytogenet 11:233-248:1984
25. Groupe Francais de Cytogenetique Hematologique: Collaborative Study of Karyotypes in Childhood Acuten Lymphoblastic Leukemias. Leukemia 7:10-19;1993.
26. Third International Workshop on chromosomes in Leukemia, 1980: Clinical significance of chromosomal abnormalities in acute lymphoblastic leukemia. Cancer Genet Cytogenet 4:111-137; 1981
27. Michael PM, Garson OM, Ekert H, Tauro G, Rennie GC, Pilkington GR: Prospective study of childhood acute lymphocytic leukemia: Hematologic, immunologic, and cytogenetic correlations. Med Pediatr Oncol 16:153-161:1988
28. Prigogina EL, Puchkova GP, Mayakova SA: Nonrandom chromosomal abnormalities in acute lymphoblastic leukemia of childhood. Cancer Genet Cytogenet 32:183-203:1988
28. Heerema NA, Palmer CG, Baehner RL: Karyotypic and clinical findings in a consecutive series of children with acute lymphocytic leukemia. Cancer Genet Cytogenet 17:165-179:1985
30. Kowalczyk JR, Grossi M, Sandberg AA: Cytogenetic findings in childhood acute lymphoblastic leukemia. Cancer Genet Cytogenet 15:47-64:1985
31. Secker-Walker LM, Alimena G, Bloomfield CD: Cytogenetics Studies of 21 Patients with Acute Lymphoblastic Leukemia in Relapse. Cancer Genet Cytogenet 40:163-169:1989
32. Heerema NA, Palmer CG, Weetman R, Bertolone S: Cytogenetic Analysis in Relapsed Childhood Acute Lymphoblastic. Leukemia 6:185-192:1992
33. Van Dongen JJM, Adriaanse HJ: Immnunobiology of leukemia. En: Henderson ES, Lister TA, Greaves MF (eds). Leukemia, Philadelphia, WB Saunders. 1996:83-130
34. Pui Ch, Crist WM: Biology and treatment of acute lymphoblastic leukemia. J Pediatr 124:491-503; 1994
35. Bloomfield CD, Peterson LC, Yunis JJ, Brunning RD: The Philadelphia chromosome (Ph1) in adults presenting with acute leukaemia: a comparison of Ph1 + and
Ph 1- patients. Br J Haematol 36:347-358; 1977
36. Rojas A, Pineda L, Gonzalez S, et al: Chromosomal abnormalities in malignant hematologic diseases. Acta Cient Venez 51:109-114;2000
37. Hermans A, Heisterkamp N, Von Lindern M, et al: Unique fusion of bcr and c-abl genes in Philadelphia chromosome positive acute lymphoblastic leukemia. Cell51:33-40:1987
38. Melo JV: The diversity of BCR-ABL fusion proteins and their relationship to leukemia phenotype. Blood 88:2375;1996
39. Paz-y-Mino C: Ecuador. En: Penchaszadeh V, editor. Medical genetic services in Latin America, New York, World Health Organization. 1998:14-16.
40. Barletta C, Pelicci P-G, Kenyon LC, Smith SD, Dalla- Favera R: Relationship between c-myb locus and the 6q- chromosomal aberration in leukemias and lymphomas. Science 235:1064-1067;1987
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Publicado
2017-06-09
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1.
Paz y Miño C, Sánchez ME, Arévalo M, Burgos R, Ruiz JC, Tapia A, Leone E. P. Estudio cromosómico en leucemias linfoblásticas agudas. Rev Fac Cien Med (Quito) [Internet]. 9 de junio de 2017 [citado 16 de abril de 2024];26(2-3):16-20. Disponible en: https://revistadigital.uce.edu.ec/index.php/CIENCIAS_MEDICAS/article/view/924
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