Identificación de kinetoplastideos aislados en el Ecuador mediante el uso de la técnica de Reacción en Cadena de Polimerasa (PCR)
Resumen
La amplificación del DNA del kinetoplasto, cada vez tiene mayor aplicación en la detección Leishmania y diagnóstico de leishmaniasis. En el presente estudio se emplea un par de sondas: KIN 1 y KIN 2, que se amplificaron en todas las especies de Leishmania, tanto las cepas de referencia como las aisladas a partir de lesiones en humanos, en quienes se observo un segmento de 470 pares de bases (pb), mientras que en Trypanosoma cruzi, un segmento de 520 pb. Es importante recalcar la necesidad de validar la utilidad de esta prueba en el diagnóstico clínico, epidemiológico y entomológico de esta enfermedad.
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1. Armijos RX, Weigel MM: Leishmaniasis. En: Panorama Epidemiológico del Ecuador. Ministerio de Salud Pública Quito, 1996.
2. Mimori T, Grimaldi G Jr, Kreutzer RD, Gómez EA, McMahon- Pratt D, Tesh RB, Hashiguchi Y: Identification, using isoenzyme electrophoresis and monoclonal antibodies, of Leishmania isolated from humans and wild animals of Ecuador. Am J Trop Med Hyg, 1989; 40: 154-158.
3. Armijos RX, Chico ME, Cruz ME, Guderian RH, Kreutzer RD, Berman JD, Grogl M: Human coetaneous leishmaniasis in Ecuador: Identification of parasites by enzyme electrophoresis. Am J Trop Med Hyg, 1990; 42: 424-428. León LA: Formas clínicas de la leishmaniasis tegumentaria americana. Pren Med Arg, 1975; 62: 73.
4. Chico ME, Guderian RG, Cooper PJ, Armijos RX, Grogl M: Evaluation of direct immunofluorecent antibodies (DIFMA) Test using Leishmania genus-specific monoclonal antibody in the routine diagnostic of coetaneous leishmaniasis. Revista de la Sociedad Brazileira de Medicina Tropical, 1995; 2: 99-103.
5. Amunarriz M: Salud y Enfermedad Patología Tropical en la Región Amazónica. Ñapo, Ecuador: CICAME, 1982; 73-78.
6. Diaz-Galarza F: Lecciones de Medicina Tropical. Universidad de Guayaquil, Ecuador, 1985; 189-218.
7. Manson-Bahr PC: Diagnosis. In the Leishmaniasis in Biology and Medicine. Ed. Peters, W & Killick-Kendrick R, 1987; 2: 703-729.
8. Hashiguchi Y, Gómez EA: A brief review of leishmaniasis in Ecuador. En: Hashiguchi Y: Studies on New World leishmaniasis and its transmission, with particular reference to Ecuador. Kochi, Japan Kyowa Printing Co. Res Rep Ser, 1987; 5-32.
9. Grimaldi G Jr, Tesh RB, Mcmahon-Pratt D: A review of the geographic distribution and epidemiology of leishmaniasis in the New World. Am J Trop Med Hyg, 1989; 41: 687-725.
10. Wirt DF, McMahon-Pratt D: Rapid Identification of leishmania species by specific hybridization of kinetoplast DNA in cutaneous lesions. Procedings of the National Academy of Science USA 1982; 79: 6999-7003.
11. Arana M, Evans D, Zolessi A Llanos A, Arévalo J: Biochemical characterízation of Leishmania (Vianna) braziliensis and Leishmania (Vianna) peruviana by isoenzymes. Transactions of the Royal Society of Tropical Medicine and Hygiene, 1990; 90: 526-529.
12. Arévalo J, Inga R, López M: PCR Detection of Leishmania braziliensis. In: Persing DH, Penover FC, Smith TF, White TJ: Diagnostic Molecular Microbiology. Principies and Applications. Washington, American Society of Microbiology, 1993; 456-461.
13. Rodgers MR, Popper SJ, Wirth DF: Amplification of kinetoplast DNA as a tool in the detection and diagnosis of Leishmania. Exp Parasitol, 1990; 71: 267-275.
14. López M, Inga R, Cueva N, Alvarez E, Arévalo J: PCR: A tool for diagnosis of American tegumentary Leishmaniasis in a health post of rural endemic areas. Archs Inst Pasteur Tunis,1993; 70(3-4): 499-504.
15. De Brujin M, Labrada L, Smyth A Santrich D, Barker D: A comparative study of diagnosis by the polymerase chain reaction and by current clinical methods using biopsies from Colombian patients with suspected leishmaniasis. Trop Med Parasitol,1993; 44:201-207.
16. Meredith S, Zijlstra E, Schoone G, Kroon C, Van Eys G, Schaeffer K, El-Hassan A, Lawyer P: Development and application of the Polymerase Chain Reaction for the detection and Identification of Leismania parasites in clinical material. Archs Inst Pasteur Tunis,
2. Mimori T, Grimaldi G Jr, Kreutzer RD, Gómez EA, McMahon- Pratt D, Tesh RB, Hashiguchi Y: Identification, using isoenzyme electrophoresis and monoclonal antibodies, of Leishmania isolated from humans and wild animals of Ecuador. Am J Trop Med Hyg, 1989; 40: 154-158.
3. Armijos RX, Chico ME, Cruz ME, Guderian RH, Kreutzer RD, Berman JD, Grogl M: Human coetaneous leishmaniasis in Ecuador: Identification of parasites by enzyme electrophoresis. Am J Trop Med Hyg, 1990; 42: 424-428. León LA: Formas clínicas de la leishmaniasis tegumentaria americana. Pren Med Arg, 1975; 62: 73.
4. Chico ME, Guderian RG, Cooper PJ, Armijos RX, Grogl M: Evaluation of direct immunofluorecent antibodies (DIFMA) Test using Leishmania genus-specific monoclonal antibody in the routine diagnostic of coetaneous leishmaniasis. Revista de la Sociedad Brazileira de Medicina Tropical, 1995; 2: 99-103.
5. Amunarriz M: Salud y Enfermedad Patología Tropical en la Región Amazónica. Ñapo, Ecuador: CICAME, 1982; 73-78.
6. Diaz-Galarza F: Lecciones de Medicina Tropical. Universidad de Guayaquil, Ecuador, 1985; 189-218.
7. Manson-Bahr PC: Diagnosis. In the Leishmaniasis in Biology and Medicine. Ed. Peters, W & Killick-Kendrick R, 1987; 2: 703-729.
8. Hashiguchi Y, Gómez EA: A brief review of leishmaniasis in Ecuador. En: Hashiguchi Y: Studies on New World leishmaniasis and its transmission, with particular reference to Ecuador. Kochi, Japan Kyowa Printing Co. Res Rep Ser, 1987; 5-32.
9. Grimaldi G Jr, Tesh RB, Mcmahon-Pratt D: A review of the geographic distribution and epidemiology of leishmaniasis in the New World. Am J Trop Med Hyg, 1989; 41: 687-725.
10. Wirt DF, McMahon-Pratt D: Rapid Identification of leishmania species by specific hybridization of kinetoplast DNA in cutaneous lesions. Procedings of the National Academy of Science USA 1982; 79: 6999-7003.
11. Arana M, Evans D, Zolessi A Llanos A, Arévalo J: Biochemical characterízation of Leishmania (Vianna) braziliensis and Leishmania (Vianna) peruviana by isoenzymes. Transactions of the Royal Society of Tropical Medicine and Hygiene, 1990; 90: 526-529.
12. Arévalo J, Inga R, López M: PCR Detection of Leishmania braziliensis. In: Persing DH, Penover FC, Smith TF, White TJ: Diagnostic Molecular Microbiology. Principies and Applications. Washington, American Society of Microbiology, 1993; 456-461.
13. Rodgers MR, Popper SJ, Wirth DF: Amplification of kinetoplast DNA as a tool in the detection and diagnosis of Leishmania. Exp Parasitol, 1990; 71: 267-275.
14. López M, Inga R, Cueva N, Alvarez E, Arévalo J: PCR: A tool for diagnosis of American tegumentary Leishmaniasis in a health post of rural endemic areas. Archs Inst Pasteur Tunis,1993; 70(3-4): 499-504.
15. De Brujin M, Labrada L, Smyth A Santrich D, Barker D: A comparative study of diagnosis by the polymerase chain reaction and by current clinical methods using biopsies from Colombian patients with suspected leishmaniasis. Trop Med Parasitol,1993; 44:201-207.
16. Meredith S, Zijlstra E, Schoone G, Kroon C, Van Eys G, Schaeffer K, El-Hassan A, Lawyer P: Development and application of the Polymerase Chain Reaction for the detection and Identification of Leismania parasites in clinical material. Archs Inst Pasteur Tunis,
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Publicado
2017-06-09
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1.
Aviles H, Armijos R, Murgueytio S, Guzmán G, Rodríguez R, McLaughIln G. Identificación de kinetoplastideos aislados en el Ecuador mediante el uso de la técnica de Reacción en Cadena de Polimerasa (PCR). Rev Fac Cien Med (Quito) [Internet]. 9 de junio de 2017 [citado 22 de julio de 2024];24(1):29-32. Disponible en: https://revistadigital.uce.edu.ec/index.php/CIENCIAS_MEDICAS/article/view/872
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