Diagnosis of Helicobacter pylori infection by C-14 urea breath test in Ecuadorian population
DOI:
https://doi.org/10.29166/rfcmq.v44i2.2690Keywords:
Helicobacter pylori, breath test, carbon-14, endoscopy, gastric biopsyAbstract
Background: Early detection of Helicobacter pylori infection has become more relevant, with urea carbon-14 (C-14) breath test as one of the non-invasive diagnostic methods; however, it has not been proven in Ecuadorian population. Objective: To evaluate the sensitivity and specificity of the C-14 urea breath test to predict HP infection in the Ecuadorian population. Methods: : Study of diagnostic tests performed on patients who attended the outpatient gastroenterology service of the Hospital Teófilo Dávila in the city of Machala, in the period April 2018 to March 2019. The patients underwent an upper endoscopy of white light (WLE) with biopsies taken to investigate HP infection. In addition, a breath test with C-14 urea was performed to compare it with the biopsies results. Results: 78 patients with a mean age of 33.76±11.2 years were studied. The diagnosis of gastritis was confirmed through endoscopy and histopathology in 100% of the patients. The breath test with urea C-14 was positive in 47/78 (60.3%) patients. Evidence of HP infection was demonstrated in gastric biopsies from 50/78 (64.1%) patients. Sensitivity, specificity, PPV, NPV, and inter-rater reliability were 94%, 100%, 100%, 90%, 96%, and 92% (P <0.001), respectively. Conclusions: The C-14 urea breath test is a useful tool for predicting HP infection in the Ecuadorian population.
Downloads
Metrics
References
Moodley Y, Linz B, Bond RP, Nieuwoudt M, Soodyall H, Schlebusch CM, et al. Age of the association between Helicobacter pylori and man. PLoS Pathog. 2012;8(5):e1002693.
Sgouras DN, Trang TT, Yamaoka Y. Pathogenesis of Helicobacter pylori infection. Helicobacter. 2015;20 Suppl 1(0 1):8-16.
Veitch AM, Uedo N, Yao K, East JE. Optimizing early upper gastrointestinal cancer detection at endoscopy. Nat Rev Gastroenterol Hepatol. 2015;12(11):660-7.
Carrillo L, Reyes R, Guerrero R, Salvador I, Montalvo R, Berger A. Ulcera duodenal, metaplasia gástrica y helicobacter pilory. Rev Fac Cien Med (Quito). 1992;17(1-4):44-50.
Sougleri IS, Papadakos KS, Zadik MP, Mavri-Vavagianni M, Mentis AF, Sgouras DN. Helicobacter pylori CagA protein induces factors involved in the epithelial to mesenchymal transition (EMT) in infected gastric epithelial cells in an EPIYA- phosphorylation-dependent manner. FEBS J. 2016;283(2):206-20.
Alarcón T, Llorca L, Perez-Perez G. Impact of the Microbiota and Gastric Disease Development by Helicobacter pylori. Curr Top Microbiol Immunol. 2017;400:253-75.
Mégraud F, Floch P, Labenz J, Lehours P. Diagnostic of Helicobacter pylori infection. Helicobacter. 2016;21 Suppl 1:8-13.
Leung WK, Wu MS, Kakugawa Y, Kim JJ, Yeoh KG, Goh KL, et al. Screening for gastric cancer in Asia: current evidence and practice. Lancet Oncol. 2008;9(3):279-87.
Darma A, Nugroho BST, Yoanna V, Sulistyani I, Athiyyah AF, Ranuh RG, et al. Comparison of Helicobacter pylori stool antigen, salivary IgG, serum IgG, and serum IgM as diagnostic markers of H. pylori infection in children. Iran J Microbiol. 2019;11(3):206-11.
Weingart V, Rüssmann H, Koletzko S, Weingart J, Höchter W, Sackmann M. Sensitivity of a novel stool antigen test for detection of Helicobacter pylori in adult outpatients before and after eradication therapy. J Clin Microbiol. 2004;42(3):1319-21.
Abd Rahim MA, Johani FH, Shah SA, Hassan MR, Abdul Manaf MR. 13C-Urea breath test accuracy for Helicobacter pylori infection in the Asian population: A meta-analysis. Ann Glob Health. 2019;85(1):110.
Jonaitis LV, Kiudelis G, Kupcinskas L. Evaluation of a novel 14C-urea breath test “Heliprobe” in diagnosis of Helicobacter pylori infection. Medicina (Kaunas). 2007;43(1):32-5.
Lopes AI, Vale FF, Oleastro M. Helicobacter pylori infection - recent developments in diagnosis. World J Gastroenterol. 2014;20(28):9299-313.
Charest M, Bélair MA. C13 urea breath test accuracy analysis against former C14 urea breath test technique: is there still a need for an indeterminate result category? J Nucl Med Technol. 2017;45(2):jnmt.116.186072
Diaconu S, Predescu A, Moldoveanu A, Pop CS, Fierbințeanu-Braticevici C. Helicobacter pylori infection: old and new. J Med Life. 2017;10(2):112-17.
Wang YK, Kuo FC, Liu CJ, Wu MC, Shih HY, Wang SS, et al.Diagnosis of Helicobacter pylori infection: Current options and developments. World J Gastroenterol. 2015;21(40):11221-35.
Hooi JKY, Lai WY, Ng WK, Suen MMY, Underwood FE, Tanyingoh D, et al. Global prevalence of Helicobacter pylori infection: systematic review and meta-analysis. Gastroenterology. 2017;153(2):420-29.
Barriga G, Arumir C, Mercado F. La prueba de aliento en el diagnóstico de la infección con Helicobacter pylori. Rev Mex Patol Clin. 2004;51(4):194-9.
Ozdemir E, Karabacak NI, Degertekin B, Cirak M, Dursun A, Engin D, et al. Could the simplified (14)C urea breath test be a new standard in noninvasive diagnosis of Helicobacter pylori infection? Ann Nucl Med. 2008;22(7):611-6.
Aguilar C, Saavedra P, Mendoza G, Bussalleu A, Cok J, Martinez F, et al. [Study of Urease Test or Breath Test (BT) and Correlation With Gastric Biopsy to Detect Helicobacter Pylori (Hp) in Dyspeptic Patients at "Hospital Nacional Cayetano Heredia Hospital" (HNCH)--Lima]. Rev Gastroenterol Peru. 2007;27(2):172-6.
Atli T, Sahin S, Arslan BU, Varli M, Yalcin AE, Aras S. Comparison of the C14 urea breath test and histopathology in the diagnosis of Helicobacter Pylori in the elderly. J Pak Med Assoc. 2012;62(10):1061-5.
Çınar A, Murat Sadıç M, Atılgan HI, Baskın A, Koca G, Demirel K, et al. Prevalence of Helicobacter Pylori Infection in School and Pre-School Aged Children With C-14 Urea Breath Test and the Association With Familial and Environmental Factors. Mol Imaging Radionucl Ther. 2015;24(2):66-70.
Ferwana M, Abdulmajeed I, Alhajiahmed A, Madani W, Firwana B, Hasan R, et al. Accuracy of urea breath test in Helicobacter pylori infection: Meta-analysis. World J Gastroenterol. 2015;21(4):1305-14.
Romagnuolo J, Schiller D, Bailey RJ. Using breath tests wisely in a gastroenterology practice: an evidence-based review of indications and pit falls in interpretation. Am J Gastroenterol. 2002;97(5):1113-26.
Munster DJ, Chapman BA, Burt MJ, Dobbs BR, Allardyce RA, Bagshaw PF, et al. The fate of ingested 14C-urea in the urea breath test for Helicobacter pylori infection. Scand J Gastroenterol. 1993;28(8):661-6.
Downloads
Published
Versions
- 2020-11-18 (2)
- 2019-09-26 (1)
How to Cite
Issue
Section
License
Copyright (c) 2020 Ronald W Albán Loayza, Alejandro S Mayorga Garcés, Wilson P Zúñiga Silva, Cristhian P Mero Chavez, Daniela G Valladarez Vázquez, Segundo A Yancha Toaza, Verónica C López Poveda
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
