Treatment of organic waste employing cellulolytic microorganisms

Abstract

The present investigation used a completely randomized experimental design (DCA), with 3 treatments (T1, T2 and T3) and a target (TB) as reference, each with 3 repetitions, with a total of 12 compost piles, located in the parish of Amaguaña. Each pile contained 132 kg of manure and rumen content from the Aldaz family slaughterhouse; 30 g of urea, 4 L of molasses and 4 Kg of sawdust; were inoculated for T1 treatment with 30 x 109 CFU of Proteus mirabilis, T2 with 30 x 109 CFU of Citrobacter freundii, T3 with 356 x 103 cells Penicillium spp. and TB without addition of microorganisms. Measurements of temperature, pH and humidity were taken 3 days after the creation of the batteries (time of adaptation of microorganisms to their new habitat). The analysis of variance (ANOVA), determined that there were no statistically significant differences between treatments, so to select the best treatment quantitative and qualitative indicators were used. As a result, T2 was the best treatment with a reduction in the amount of organic waste of 80,97%; a Tmax of 46 °C, organic matter of 80,02%, producing a highly saline dark brown organic substrate with pH 9.04, total nitrogen (4%), total phosphorus (0,02%), potassium (0,73%) and free of fecal pathogens.