Detection of mycotoxins (aflatoxins) in primary and processed foods for humans and farm animals, in Riobamba-Ecuador
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Abstract
Mycotoxins are substances produced by several species of molds that can grow on food under certain conditions of humidity and temperature, and constitute a real risk to human and animal health. The objective of the present study was to determine the presence of Mycotoxins (Aflatoxins) in primary and processed foods for humans and farm animals in Riobamba, Ecuador. Twelve samples were taken with 4 replicates of primary foods: rice, chicken, raw milk, corn, alfalfa, and wheat; and, processed foods: chicken sausages, pasteurized milk, bread and concentrates for chickens, cows, and pigs from the different markets with the highest concentration of product offer, which were analyzed through the ELISA enzyme-linked immunosorbent method (Veratox® for Aflatoxin Quantitative Test and Veratox® Aflatoxin M1, NEOGEN) with the capacity to determine quantitatively aflatoxins B1, B2, G1, G2, M1. Of the total samples analyzed, 98 % presented contamination by aflatoxins, which according to the Ecuadorian Food Codex are within the allowed permitted parameters of less than 10 µg kg-1, for food and feed, and, less than 0.5 µg l-1 for milk.
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References
Abdel-Wahhab, M. A., Omara, E. A., Abdel-Galil, M. M., Hassan, N. S., Nada, S. A., Saeed, A., y el-Sayed, M. M. (2007). Zizyphus spina-christi extract protects against aflatoxin B1-initiated hepatic carcinogenicity. African journal of traditional, complementary, and alternative medicines, 4(3), 248-256. https://journals.athmsi.org/index.php/ajtcam/article/view/216
Alexander, J., Benford, D., Cockburn, A., Cravedi, J.-P., Dogliotti, E., Domenico, A. di, Luisa Fernández-Cruz, M., Fink-Gremmels, J., Fürst, P., Galli, C., Grandjean, P., Gzyl, J., Heinemeyer, G., Johansson, N., Mutti, A., Schlatter, J., Leeuwen, R. van, Peteghem, C. van, y Verger, P. (2009). Effects on public health of an increase of the levels for aflatoxin total from 4 µg/kg to 10 µg/kg for tree nuts other than almonds, hazelnuts and pistachios - Statement of the Panel on Contaminants in the Food Chain. EFSA Journal, 7(6), 1168. https://doi.org/10.2903/J.EFSA.2009.1168
Alshannaq, A., y Yu, J. H. (2017). Occurrence, Toxicity, and Analysis of Major Mycotoxins in Food. International journal of environmental research and public health, 14(6), 632. https://doi.org/10.3390/ijerph14060632
Bennett, J. W., y Klich, M. (2003). Mycotoxins. Clinical Microbiology Reviews, 16(3), 497-516. https://doi.org/10.1128/CMR.16.3.497-516.2003
Bogantes-Ledezma, P., Bogantes-Ledezma, D., y Bogantes-Ledezma, S. (2004). Aflatoxinas. Acta Médica Costarricense, 46(4), 174-178. https://doi.org/10.51481/amc.v46i4.157
Dhakal, A., Hashmi, M. F., y Sbar, E. (2022). Aflatoxin Toxicity. En StatPearls. StatPearls Publishing.
Grimaud, T., y Jhonson, J., (2009). Microorganisms in raw Milk.
Hamzeh Pour, S., Mahmoudi, S,. Masoumi, S., Rezaie, S., Barac, A.,Ranjbaran, M., Oliya, S., Mehravar, F., Sasani, E., Noorbakhsh, F., y Khodavaisy, S. (2019). Aflatoxin M1 contamination level in Iranian milk and dairy products: A systematic review and meta-analysis. World Mycotoxin Journal, 13(1), 67-82. https://doi.org/10.3920/WMJ2019.2485
International Agency for Research on Cancer [IARC]. (1993). Some Naturally Occurring Substances: Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans Volume 56. IARC. https://publications.iarc.fr/Book-And-Report-Series/Iarc-Monographs-On-The-Identification-Of-Carcinogenic-Hazards-To-Humans/Some-Naturally-Occurring-Substances-Food-Items-And-Constituents-Heterocyclic-Aromatic-Amines-And-Mycotoxins-1993
Iram, W., Anjum, T., Iqbal, M., Ghaffar, A., y Abbas, M., (2016). Structural elucidation and toxicity assessment of degraded products of aflatoxin B1and B2 by aqueous extracts of Trachyspermum ammi. Frontiers in Microbiology, 7, 346. https://doi.org/10.3389/fmicb.2016.00346
Kępińska-Pacelik, J., y Biel, W. (2021). Alimentary risk of mycotoxins for humans and animals. Toxins, 13(11), 822. https://doi.org/10.3390/toxins13110822
Landeros, P., Noa, M., López, Y., González, D. G., Noa, E., Real, M., Juárez, C., y Medina, M. S. (2012). Niveles de Aflatoxina M1 en leche cruda y pasteurizada comercializada en la zona metropolitana de Guadalajara, México. Revista de Salud Animal, 34(1), 40-45. http://revistas.censa.edu.cu/index.php/RSA/article/view/71
Murshed, S. (2020). Evaluation and Assessment of Aflatoxin M1 in Milk and Milk Products in Yemen Using High-Performance Liquid Chromatography. Journal of Food Quality, 2020, 8839060. https://doi.org/10.1155/2020/8839060
Nakai, V. K., de Oliveira Rocha, L., Gonçalez, E., Fonseca, H., Ortega, E. M. M., y Corrêa, B. (2008). Distribution of fungi and aflatoxins in a stored peanut variety. Food Chemistry, 106(1), 285-290. https://doi.org/10.1016/J.FOODCHEM.2007.05.087
Neogen. (2014). Veratox® para aflatoxina. Micotoxinas. https://www.neogen.com/es/categories/mycotoxins/veratox-aflatoxin/
NTE INEN 9:2015. Leche cruda. Requisitos. Sexta revisión. https://www.normalizacion.gob.ec/buzon/normas/nte_inen_009_6r.pdf
Payne, G. A., Nystrom, G. J., Bhatnagar, D., Cleveland, T. E., y Woloshuk, C. P. (1993). Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus. Applied and environmental microbiology, 59(1), 156-162. https://doi.org/10.1128/aem.59.1.156-162.1993
Prandini, A., Tansini, G., Sigolo, S., Filippi, L., Laporta, M., y Piva, G. (2009). On the occurrence of aflatoxin M1 in milk and dairy products. Food and Chemical Toxicology, 47(5), 984-991. https://doi.org/10.1016/j.fct.2007.10.005
R Core Team. (2021). R: A language and environment for statistical computing. R Foundation for Statistical Computing, https://www.R-project.org/
Rahimi, A., Sasani, E., Rezaie, S., Soltan Dallal, M. M., Mahmoudi, S., Ahmadi, A., Ghaffari, M., Aala, F., y Khodavaisy, S. (2021). Molecular identification of aflatoxigenic Aspergillus species in dried nuts and grains collected from Tehran, Iran. Journal of Environmental Health Science and Engineering, 19(2), 1795-1799. https://doi.org/10.1007/s40201-021-00734-6
Reglamento (CE) N° 1881/2006 de la Comisión de 19 de diciembre de 2006 por el que se fija el contenido máximo de determinados contaminantes en los productos alimenticios. Versión consolidada actual: 01/07/2022. http://data.europa.eu/eli/reg/2006/1881/2022-07-01
Reglamento (EU) N° 165/2010 de la Comisión, de 26 de febrero de 2010, que modifica, en lo que respecta a las aflatoxinas, el Reglamento (CE) n o 1881/2006 por el que se fija el contenido máximo de determinados contaminantes en los productos alimenticios. https://eur-lex.europa.eu/legal-content/ES/ALL/?uri=CELEX:32010R0165
Romani, L. (2004). Immunity to fungal infections. Nature Reviews Immunology, 4, 11-24. https://doi.org/10.1038/nri1255
Sandoval Cañas, G. J. (2013). Determinación de aflatoxinas totales, por cromatografía líquida de alta resolución (HPLC), en matriz de cereales: maíz y cebada. Universidad Central del Ecuador. http://www.dspace.uce.edu.ec/handle/25000/2159
Signorell, A., et al. (2021). DescTools: Tools for descriptive statistics. R package version 0.99.44. https://cran.r-project.org/package=DescTools