Efeito antifúngico de diferentes concentrações hidroalcoólicas de Uncaria Tomentosa em Candida albicans: Estudo in vitro
DOI:
https://doi.org/10.29166/odontología.vol19.n2.30-39Keywords:
Uña de Gato (cat’s claw), Oral health, Mycosis, Medicinal plantsAbstract
At present, the use of phytotherapy is in greater demand by patients and many health professionals due to its easy access, low cost and because of adverse reactions. Objective: To determine the antifungal effect of different hydroalcoholic concentrations of Uncaria tomentosa (UT) about Candida albicans ATCC 10231, Materials and methods: In vitro experimental study. The sample consisted of 24 box of petri dishes with Sabourand agar, each with six discs corresponding to the 6 study groups being: G1 Nistatin 21 ul (Positive Control); G2 hydroalcoholic extract of 100% UT; G3 hydroalcoholic extract of 75% UT; G4 hydroalcoholic extract of 50% UT; G5 hydroalcoholic extract of 25% UT; G6 70 ° Ethyl Alcohol (Negative Control) A hydroalcoholic extract was elaborated by maceration, using 75mg of micropulverized plant and 250ml of ethyl alcohol of 70 °, 150ml of extract was obtained, the same one that was diluted to obtain three secondary concentrations at 75%, 50% and 25%. The data were processed and analyzed through the ANOVA and Bonferroni test with a level of significance of 5%. Results: A halo of inhibition of 6.46 mm, 10.96 mm, 14.75 mm and 16.5 mm was observed for the hydroalcoholic extracts at 25%, 50%, 75% and 100% respectively. Nistatin showed a halo of inhibition of 23.42 mm and ethyl alcohol of 70 ° did not have any antifungal effect. A statistically significant difference was observed between the groups (<0.001). Conclusion: The 100% hydroalcoholic extract of UT such as nystatin showed to be sensitive, while the concentrations at 50% and 75% showed intermediate sensitivity and the concentration at 25% was resistant against Candida albicans.
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