Study of the antimicrobial properties of Camellia sinensis in an oral microbial model
Keywords:
Phytotherapy, Streptococcus mutans, Candida AlbicansAbstract
Objective: To determine the in vitro antibacterial effect of the extract of Camellia Sinensis (green tea) on an oral microflora model. Materials and methods: Two ethanolic green tea extracts of different origin (Peru and China) were used in concentrations of 16, 8, 4, 2, 1, 0.5 and 0.25mg / ml. The minimum and maximum inhibitory concentrations were evaluated on S. mutans, S. mitis, C. albicans, C. tropicalis, S. aureus, F. nucleatum, L. rhamnosus, L. casei, C. glabrata and E. faecalis strains. The obtained data were statistically analyzed using the Kruskal Wallis statistical test with a significance level of 5%. Results: C. sinensis showed a bacteriostatic effect for the strains of S. mutans, and fungistatic for C. albicans, C. tropicalis and C. glabrata. (P <0.05). Conclusion: The 16mg / ml ethanolic extract of Camellia Sinensis has an inhibitory effect on strains of S. mutans, C. albicans, C. tropicalis and C. glabrata.
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Piatkowska E, Rusiecka-Ziolkowska J. Influence of Essential Oils on Infectious Agents. Adv Clinical Exp. 2016; 25(5):989-95.
Anita P, Sivasamy S, Madan Kumar PD, Balan IN, Ethiraj S. In vitro antibacterial activity of Camellia sinensis extract against cariogenic microorganisms. J Basic Clin Pharm. 2014; 6(1):35-9.
Araghizadeh A, Kohanteb J, Fani MM. Inhibitory activity of green tea (Camellia sinensis) extract on some clinically isolated cariogenic and periodontopathic bacteria. Med Princ Pract. 2013;22(4):368-72.
Sicca C, Bobbio E, Quartuccio N, Nicoló G, Cistaro A. Prevention of dental caries: A review of effective treatments. J Clinical Exp Dent. 2016; 8(5):e604-e10.
Legenova K, Bujdakova H. [The role of Streptococcus mutans in the oral biofilm]. Epidemiologie, mikrobiologie, imunologie: casopis Spolecnosti pro epidemiologii a mikrobiologii Ceske lekarske spolecnosti JE Purkyne. 2015;64(4):179-87.
Espinoza MS, León RAM. Prevalencia y experiencia de caries dental en estudiantes según faet cultades de una universidad particular peruana. Rev Estomatol Herediana. 2015; 25(3):187-93.
Duarte MC, Figueira GM, Sartoratto A, Rehder VL, Delarmelina C. Anti-Candida activity of Brazilian medicinal plants. Journal of ethnopharmacology. 2005;97(2):305-11.
Steinmann J, Buer J, Pietschmann T, Steinmann E. Anti-infective properties of epigallocatechin-3-gallate (EGCG), a component of green tea. Br J Pharmacol. 2013;168(5):1059-73.
Ricardo PMP. Efecto antibacteriano in vitro del extracto de té verde (camellia sinensis) en comparación con el gluconato de clorhexidina al 0.12% sobre el streptococcus mutans en el laboratorio microbiológico de la UCSM, 2011.: Universidad Católica de Santa María; 2011.
Ferrazzano GF, Roberto L, Amato I, Cantile T, Sangianantoni G, Ingenito A. Antimicrobial properties of green tea extract against cariogenic microflora: na in vitro study. J Med Food. 2011; 14(9): 907-11.
Awadalla HI, Ragab MH, Bassuoni MW, Fayed MT, Abbas MO. A pilot study of the role of green tea use on oral health. Int J Dent Hyg. 2011; 9(2): 110-6.
Antunes DP, Salvia AC, de Araújo RM, Di Nicoló R, Koga Ito CY, de Araujo MA. Effect of green tea extracted and mouthwash without alcohol on Candida albicans biofilm on acrylic resin. Gerodontology. 2015; 32(4): 291-5.