Actinomyces israelli bacterial reduction using ozonated water
Keywords:
Ozonated water, sodium hypochlorite, Actinomyces israelii, apical periodontitisAbstract
Actinomyces israelii, a pathogenic microorganism of periapical tissues, is difficult to eradicate due to its need of few nutrients and oxygen to subsist, thus developing an apical periodontitis. Objective: Compare 5% ozonated water, 5.25% sodium hypochlorite and 2% chlorhexidine bactericidal effect on Actinomyces israelii Materials and methods: 40 human uniradicular root canals were inoculated with Actinomyces israelii. After irrigation with the different solutions, bacterial reduction was measured through a UFC/Plaque count. The 40 teeth were divided into 4 groups: G1: Physiological serum, G2: 5% ozonated water, G3: 2% Chlorhexidine G4: 5.25% Sodium hypochlorite. The Tukey statistical test significance level was 5%. Results: The Tukey test evidenced 0 average values for 5.25% sodium hypochlorite, 0 for 2% chlorhexidine and 0.3 for ozonated water. This test determined that 5.25% NaOCl is the first choice treatment followed by 2% chlor-hexidine, and 5% ozonated water. No significant differences were found on the ozonated water action and other solutions (<0.15). Conclusions: 5% ozonized water was effective in the bacterial reduction of A. israelli as well as 5.25% NaOCl, without significant statistical differences.
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References
Siqueira JF, Rocas IN. Microbiología endodóncica. In Torabinejad M. Endodoncia, Principios y Práctica. Es-paña: Elseiver; 2010. p. 38 - 4
Sundqvist G, Figdor D. Life as an endodontic patho-gen. Ecological differences between the untreated and root-filled root canals. Endodontic Topics. 2003; 6(1).3-28.
Piovano S. Microbiología de las enfermedades gingi-voperiodontales, de la periimplantitis, de los conductos radiculares y de los procesos perirradiculares. In Ne-groni N. Microbiología Estomatológica: Fundamentos y Guía Práctica. Buenos Aires: Médica Panamericana; 2009. p. 319-324.
Jhajharia K, Parolia A, Vikram SK. Biofilm in endo-dontics: A review. Journal of International Society of Preventive & Community Dentistry. 2015; 5(1). 1-12.
Radcliffe CE, Potouridou L, Qureshi R, Habahbeh N, Qualtrough A, Worthington H, et al. Antimicrobial acti-vity of varying concentrations of sodium hypoclorite on the endodontic microorganisms Actinomyces israelii, A. naeslundi, Candida albicans and Enterococcus faecalis. International Endodontic Journal. 2004; 37(7). 438-46.
Tessier J. El ozono en odontología. Dental Tribune. 2014; 2(1). 21-2.
Monzavi AE. In vitro and ex vivo antimicrobial effica-cy of nano-MgO in the elimination of endodontic pa-thogens. Clin Oral Investing. 2014; 19(2). 56.
Recai Z, Gizem K, Ihsan H, Zeynep S, Tutku T, Zuhal M. Bactericidal effects of various irrigation solutions against Staphylococcus Aureus in human root canal. Journal Istanbul Univ. Fac. Dent. 2015; 49(1). 19-24.
Ochoa JD, Rendon JA, Morales S, Ferrer ME. Redi-CES. [Online].; 2016 [cited 2016 abril 13. Available from: http://hdl.handle.net/10946/4091.
Cardoso M, Dias L, Yumi C, Cardoso AO. Effective-ness of ozonated water on Candida albicans, Entero-coccus faecalis, and endotoxins in root canals. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiolo-gy. 2008; 105(3). 85-91.
Huth KC, Quirling M, Maier S, Kamereck K, Alkhayer M, Paschos E. Effectiveness of ozone against endodon-topathogenic microorganisms in a root canal biofilm model. International Endodontic Journal. 2009; 42(1). 13.
