Cyclical fatigue of four reciprocating systems in a dynamic dump of simulating roots

Authors

DOI:

https://doi.org/10.29166/odontologia.vol22.n2.2020-33-44

Keywords:

Nanoparticles, antimicrobial disc diffusion tests, mutans streptococci, microbiology, orthodontics, in vitro techniques

Abstract

The fixed appliances used in orthodontics make proper oral hygiene difficult, in these conditions the development of carious lesions can be a complication, one of the main etiologic agents involved is Mutans streptococci. It was determined that silver nanotechnology has an important preventive application in dental biomaterials. Objective: Antimicrobial activity on Mutans streptococci of silver nanoparticles (AgNPs) included in orthodontic adhesives at different concentrations. Materials and methods: In 20 Petri dishes with Mutans streptococci inoculated in Müller-Hinton agar supplemented with 5% blood, 6 paper discs were placed, 4 soaked up with AgNPs included in an orthodontic adhesive at concentrations of 25, 75, 125 and 175 ppm, and 2 control discs with 0.12% chlorhexidine and distilled water. Antimicrobial capacity was determined based on averages of inhibition halos at 48 hours of incubation, compared to the Duraffourd scale and 0.12% chlorhexidine. Results: At 25 ppm there was no antibacterial capacity, at 75 ppm 50% reached limit sensitivity, but 100% of the samples at 125 and 175 ppm achieved limit sensitivity, however, chlorhexidine 0.12% showed higher sensitivity between average 60% and highly sensitive 40%. Conclusión: Silver nanoparticles smaller than 100 nm, included at 125 and 175 ppm in orthodontic adhessives, have antimicrobial activity on Strptococcus mutans, however, when studying, they don’t reach the sensitivity of chlorhexidine at 0.12%.

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Author Biographies

Luis Martin Guevara Ruiz

Graduado de la Carrera de Odontología de la Universidad Central del Ecuador; Quito, Ecuador.

Pablo Mauricio Bonilla Valladares

Bioquímica Farmacéutica, Universidad Central del Ecuador. Master en Educación Superior y Administración Educativa. Universidad Indoamérica. Docente de la Carrera de Odontología de la Universidad Central del Ecuador; Quito, Ecuador.

María Fernanda Caicedo Breedy

Químico, Universidad Central del Ecuador. Master en Nanociencia y Nanotecnología, Universidad de Barcelona, Docente de la Carrera de Química de la Universidad Central del Ecuador; Quito, Ecuador.

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Published

2020-07-01

How to Cite

Guevara Ruiz, L. M., Bonilla Valladares, P. M., & Caicedo Breedy , M. F. (2020). Cyclical fatigue of four reciprocating systems in a dynamic dump of simulating roots. Odontología, 22(2), 33–44. https://doi.org/10.29166/odontologia.vol22.n2.2020-33-44

Issue

Section

Scientific article