Antibacterial effectiveness between passive and continuous ultrasonic irrigation system on Enterococcus faecalis. In vitro study
DOI:
https://doi.org/10.29166/odontologia.vol23.n2.2021-e3439Keywords:
Biofilm, Enterococcus Faecalis, Duct Treatment, Complementary DisinfectionAbstract
To be successful in endodontic therapy, root canal disinfection is very important. Objective. To compare the antibacterial effectiveness between the Passive Ultrasonic Irrigation System and the Continuous Ultrasonic Irrigation on a mature Enterococcus faecalis biofilm. Methodology. Experimental in vitro study. The sample consisted of 30 teeth, single-rooted premolars, which were standardized to a length of 16mm. The experimental units were subjected to an autoclaving process, and then strains of Enterococcus faecalis ATCC 29212 were cultivated by placing approximately 0.01 mL of standard value of the bacterial suspension in each root canal until obtaining mature biofilm for 21 days. The chemical-mechanical preparation was carried out by means of manual instrumentation with files from the universal Protaper system, following the manufacturer’s instructions until reaching the Protaper F3 file. During and after the use of each instrument, the canals were irrigated with 2mL of 2.5% NaOCl with Navitip 30-G needles. Root canal samples were collected with sterile #15 paper cones, before mechanical-chemical preparation (S1), after mechanical-chemical preparation (S2) and after the final irrigation protocol (S3). After (S2) the sample was divided into two groups: G1 (n=10) application of Passive Ultrasonic Irrigation, and G2 (n=10) application of Continuous Ultrasonic Irrigation, in addition to a positive control group and a negative control group. The samples obtained were seeded in Müller-Hinton Agar and incubated for 48 hours and subsequently the colony-forming units (CFU) were counted. The data obtained were processed in the statistical program SPSS. Analysis of results. The Shapiro-Wilk Normality test was carried out, in which the values of the level of significance (Sig) were higher than 0.05 (95% reliability); therefore, the samples come from populations with Normal distribution, Parametric tests were used to compare groups: ANOVA, Student’s T. Differences between the processes were obtained as a result: Chemical-mechanical preparation eliminated an average of 93.9% of mature Enterococcus faecalis biofilm, while passive ultrasonic activation eliminated 98.9% and continuous ultrasonic activation eliminated 99.2%. Therefore, the complementary disinfection techniques used showed significant differences compared to the chemical-mechanical preparation (p=0.000). Passive Ultrasonic Activation does not show significant differences (p = 0.393) compared to Continuous Ultrasonic Activation. With these results, it is concluded that the two complementary activation systems turn out to have the same antibacterial efficacy on Enterococcus faecalis.
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Copyright (c) 2021 Jessica Fernanda Ibarra de la Vega, José Eduardo Maldonado Paredes, Laura Cristina Nardello Leite, Roberto Xavier Romero Cazares
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