Antibacterial effect of Procaine 2% with caffeine and of propolis 40% on strains of Enterococcus faecalis: in vitro study
Keywords:
Phytotherapeutic agents, bacterial reduction, irrigant in endodonticAbstract
The use of phytotherapeutic agents in health areas has become increasingly important thanks to its antibacterial capabilities and considerable biocompatibility. Objective: to evaluate the antibacterial effect of procaine 2 % with caffeine and propolis 40 %, against Enterococcus faecalis strains, as an enhancer for irrigation in root canal treatment. Materials and methods: the sample consisted of 28 Petri dishes with a culture medium of lamb blood agar. Strains of Enterococcus faecalis ATCC 29212 were cultured in each dish. White Sensi discs soaked in the mentioned substances were added: G1 – Sodium Hypochlorite with a 5 % concentration positive control; G2 - G2: propolis 40%, G3: Procaine 2 % concentration and caffeine. G2: Propóleo 40%, G3: Procaine 2% with caffeine 0,25% (Impletol) and G4: Saline Solution control negativo. The samples were cultured in an anaerobic environment and were incubated at 35°C, the diameter of the bacterial inhibition area was measured after 48 hours. The data recollected were statistically analyzed with the Turkey Test with a significance level of 5%. Results: the value of the average of inhibition halos for each group was: (G1)21,54;(G2)10,18mm and 0,0mm for G3 and G4. There were statistically significant differences between the substances used with the sodium hypochlorite (p<0,05). Conclusions: the ptopolis is a adjuvant substance in the root canal treatment irrigation while the procaine with caffeine didvnot show any antibacterial effect on E. faecalis.
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References
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33. Esimone CO; Okoye FBC; Nworu CS; Agubata CO. In vitro interaction between caffeine and some penicillin antibiotics against Staphylococcus aureus. Tropical Journal of Pharmaceutical Research. 2008;7(2):969-74.
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35. Kayaoglu G, Orstavik D. Virulence factors of Enterococcus faecalis: relationship to endodontic disease. Crit Rev Oral Biol Med. 2004;15(5):308-20.
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2. Jean-Prost P, Le Conte Y. Apicultura Conocimento de la abeja. Manejo de la colmena. 4ta ed. Madrid: Ediciones mundi-Prensa; 2007.
3. Siqueira JF, Jr., Alves FR, Versiani MA, Rocas IN, Almeida BM, Neves MA, et al. Correlative bacteriologic and micro-computed tomographic analysis of mandibular molar mesial canals prepared by self-adjusting file, reciproc, and twisted file systems. J Endod. 2013;39(8):1044-50.
4. Versiani MA, De-Deus G, Vera J, Souza E, Steier L, Pecora JD, et al. 3D mapping of the irrigated areas of the root canal space using micro-computed tomography. Clin Oral Investig. 2015;19(4):859-66.
5. Amoroso-Silva P, Alcalde MP, Hungaro Duarte MA, De-Deus G, Ordinola-Zapata R, Freire LG, et al. Effect of finishing instrumentation using NiTi hand files on volume, surface area and uninstrumented surfaces in C-shaped root canal systems. Int Endod J. 2017;50(6):604-11.
6. Ordinola-Zapata R, Bramante CM, Garcia RB, de Andrade FB, Bernardineli N, de Moraes IG, et al. The antimicrobial effect of new and conventional endodontic irrigants on intra-orally infected dentin. Acta Odontol Scand. 2013;71(3-4):424-31.
7. Zehnder M. Root canal irrigants. J Endod. 2006;32(5):389-98.
8. Haapasalo M, Shen Y, Qian W, Gao Y. Irrigation in endodontics. Dent Clin North Am. 2010;54(2):291-312.
9. Nair PN, Henry S, Cano V, Vera J. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after "one-visit" endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005;99(2):231-52.
10. Nair PN. Pathogenesis of apical periodontitis and the causes of endodontic failures. Crit Rev Oral Biol Med. 2004;15(6):348-81.
11. Chávez de Paz L. Redefining the Persistent Infection in Root Canals: Possible Role of Biofilm Communities. Journal of Endodontics, 2007;33(6):652-62.
12. Pinheiro ET; Gomes BPFA; Druker DB; Zaia AA; Ferraz CCR; Souza-Filho FJ. Antimicrobial suceptibility of Enterococcus faecalis isolated from canals of root filled teeth with periapical lesions. International Endodontic Journal, 2004;37:756-63.
13. Zehnder M. Root Canal Irrigants. Journal of Endodontics, 2006; 32(5):389 –98
14. Weinsten MP. Comparative Evaluation of Penicilin, Ampicilin and Imipenem MICs ans Susceptibility Breakpoints for Vancomycin- Susceptible and Vancomycin-Resistant Enterococcus faecalis and Enterococcus faecium. Journal of Clinical Microbiology, 2001;39(7):2729-31.
15. Panaque MR; Gonzalez AM; Rodriguez BM. Utilidad de la terapia neural en sepsis de conductos radiculares 2008 - 2009. Policlínico tipo III. Multimed, 2009;13(3-4).
16. Morse D. Immunologic aspects of pulpal- periapical diseases: A review. Oral Surgery, Oral Medicine, Oral Pathology, 1977;43(3):436-51.
17. CLSI. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard—Eleventh Edition. CLSI document M02-A11. Wayne, PA: Clinical and Laboratory Standards Institute. 2012;29(1).
18. Koo H; Rosalen PL; Cury JA; Park YK; Bowen WH. Effects of compounds found in propolis on Streptococcus mutans growth and on glucosyltransferase activity. Antimicrob Agents Chemother, 2002;46(5):1302-9.
19. Gutiérrez MS; Romero GC; Hidalgo GCR; Pérez CEO; Díaz PB. Acción antibacteriana de la tintura hidroalcohólica de propóleos al 4 % en gérmenes de origen endodóncico. AMC;1999,3(4).
20. Ferreira FB; Torres SA; Rosa OP; Ferreira CM; Garcia RB; Marcucci MC; Gomes BP. Antimicrobial effect of propolis and other substances against selected endodontic pathogens. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2007;104(5):709-16.
21. Gonzalón López, Lilian Patricia. Estudio comparativo in vitro del efecto antibacteriano como medicamento intraconducto entre el hidróxido de calcio y el extracto de propóleo sobre el enterococcus faecalis, [Tesis de pregrado]. Quito: Univerisidad Central del
Ecuador, Facultad de Odontología;2015
22. Soon, Ji. Estudio comparativo in vitro de la actividad antimicrobiana de Propóleo versus Hipoclorito de Sodio utilizados como irrigantes en Endodoncia. [Tesis de Posgrado de Endodoncia]. Quito: Univerisidad Central del Ecuador, Facultad de Odontología;2013
23. Arslan S; Ozbilge H; Kaya EG; Er O. In vitro antimicrobial activity of propolis, BioPure MTAD, sodium hypochlorite, and chlorhexidine on Enterococcus faecalis and Candida albicans. Saudi medical journal, 2011;32(5):479-83.
24. Moncla BJ; Guevara PW; Wallace JA; Marcucci MC; Nor JE; Bretz WA. The inhibitory activity of typified propolis against Enterococcus species. Z Naturforsch, 2012;67(5-6):249-56.
25. Drago L; Mombelli B; De Vecchi E; Fascina MC; Tocalli L; Gismondo M. In Vitro Antomicrobial Activity of propolis dry extract. J Chemoterapy, 2000;12(5):390-5.
26. Maia Filho E; Maia C; Bastos A; Novais T. Efeito antimicrobiano in vitro de diferentes medicações endodônticas e própolis sobre Enterococcus faecalis. RGO, 2008; 56(1):21-5.
27. Daglia M. Polyphenols as antimicrobial agents. Current Opinion in Biotechnology, 2012;23(2):174-81.
28. Cushnie TP; Lamb A. Recent advances in understanding the antibacterial properties of flavonoids. International Journal of Antimicrobial Agents. 2011, 38(2):99-107.
29. Petti S; Scully C. Polyphenols, oral health and disease: A review. Journal of Dentistry. 2009;37(6):413–23.
30. Van der Waal S; Oonk C; Nieman S; Wasselink P; De Soet J; Crielaard W. Additional disinfection with a modified salt solution in a root canal model. Journal of Dentistry. 2015;43(10):1280–84.
31. Machiavelli R. La Terapia Neural 2006 Disponible en: http://www.terapianeural.com.
32. Ferrazzano GF; Amato I; Ingenito A; De Natale A; Pollio A. Anti-cariogenic effects of polyphenols from plant stimulant beverages (cocoa, coffee, tea). Fitoterapia. 2009;80(5):255-62.
33. Esimone CO; Okoye FBC; Nworu CS; Agubata CO. In vitro interaction between caffeine and some penicillin antibiotics against Staphylococcus aureus. Tropical Journal of Pharmaceutical Research. 2008;7(2):969-74.
34. Almeida AA; Farah A; Silva DA; Nunan EA; Gloria MB. Antibacterial activity of coffee extracts and selected coffee chemical compounds against enterobacteria. J Agric Food Chem. 2006;54(23):8738-43.
35. Kayaoglu G, Orstavik D. Virulence factors of Enterococcus faecalis: relationship to endodontic disease. Crit Rev Oral Biol Med. 2004;15(5):308-20.
36. Zhang C, Du J, Peng Z. Correlation between Enterococcus faecalis and Persistent Intraradicular Infection Compared with Primary Intraradicular Infection: A Systematic Review. J Endod. 2015;41(8):1207-13
Published
2018-10-18
How to Cite
Barragán Chaguaro, T. G., & Romero Cazares, R. X. (2018). Antibacterial effect of Procaine 2% with caffeine and of propolis 40% on strains of Enterococcus faecalis: in vitro study. Odontología, 19(1), 110–121. Retrieved from https://revistadigital.uce.edu.ec/index.php/odontologia/article/view/1105
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Section
Scientific article
