Inhibitory effect of the essential oil of Cymbopogon Citratus on the strain of Porphyromona Gingivalis: In Vitro Study

Abstract

Plants with therapeutic properties used in traditional healthcare have been studied in recent years and are an important alternative to different dental treatments. Objective: To determine the inhibitory effect of the essential oil of Cymbopogon Citratus (CC) at different levels on the Porphyromonas Gingivalis (Pg) strain. Materials and methods: Experimental study, in vitro. The sample consisted of 24 Petri dishes with cultures of Pg ATCC® 33277 ™ in Mueller Hinton Agar, each with 5 discs corresponding to the 5 study groups being: G1 essential oil of 100% CC; G2 50% CC essential oil; G3 75% CC essential oil; G4 chlorhexidine 0.12% (positive control); G5 physiological saline (negative control). At 24 hours of incubation, the halos are measured using the Antibiotic Zone Scale (mm) with the sensitivity parameters determined by the Duraffourd scale et al, 1986 being: Null (-) <8 mm, Sensitive (+) > 8 mm ≤14 mm, Very sensitive (++) > 14 ≤ 20mm and Extremely sensitive (+++)> 20mm. It was used for the ANOVA and Tukey tests analysis with a level of significance of 5%. Results: The CC essential oil showed a halo of inhibition of 14 mm, 12 mm and 10 mm at concentrations of 100%, 50% and 75% respectively. Chlorhexidine 0.12% haunted values ​​of 17mm inhibition. There was a statistically significant difference between the groups (<0.001). Conclusion: The essential oil of CC 100%, 50% and 75% proved to be sensitive, against the Porphyromona Gingivalis.