Molecular diagnosis of microorganisms periodontopathogens in alcoholic - smoking patients with chronic periodontitis of the city of Loja, Ecuador

Authors

  • Alexandra Johanna Aguilar Betancourt Odontóloga por la Facultad de Odontología de la Universidad Nacional de Loja. Egresada del Postgrado de Rehabilitación Oral de la Universidad Central del Ecuador
  • Gustavo Tello PhD en Odontopediatria por la Facultad de Odontología de la Universidad de São Paulo – Brasil; (FOUSP). Profesor-investigador coordinador de investigación del posgrado de la Facultad de Odontología; Universidad Central del Ecuador
  • Loidy Zamora Gutierrez Docente investigadora, Centro de Biotecnología; Universidad Nacional de Loja
  • Susana Patricia González Eras Odontóloga Especialista en Odontopediatria Universidad Central del Ecuador, Docente de la Carrera de Odontología; Universidad Nacional de Loja

DOI:

https://doi.org/10.29166/odontologia.vol20.n1.2018-33-49

Keywords:

Periodontics, Polymerase Chain Reaction, Porphyromona gingivalis, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans

Abstract

Alcoholic and smoking patients have a greater predisposition to develop periodontal disease. Objective: To determine the presence of microorganisms periodontopathogens: Porphyromona gingivalis, Treponema denticola, Tannerella forsythia, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans by Polymerase Chain Reaction (PCR), in alcoho­lic-smoking patients with chronic periodontitis. Materials and methods: The group was composed of 31 patients between the ages of 18 to 62-year-old, male alcoholics and/or smokers in which were excluded those who take some kind of drug, giving a sample of 23 patients with periodontal pockets ≥ 6mm from the Center of Rehabilitation of the city of Loja, Ecuador. A full periodontal examination and the sample taking were in two of the most profound sites of each patient. The variables analyzed were: the presence of dental biofilm, bleeding on probing, probing depth and clinical attachment level. The data were analyzed with the Kruskal Wallis Test with a significance level of 5%. Results: The chronic periodontitis was in the 52.1% of the patients between the ages of 18 to 30 years old, is more susceptible in risky alcoholics-smokers and low smokers. The 91.04 % of alcoholics and smokers are associated with the presence of dental biofilm (p = 0.028) and in the molecular diagnosis the 41.18% of patients exhibit more than 3 microorganisms (p = 0.039). Conclusion: That observed presence of the periodontopathogens in alcoholic-smoking patients with chronic periodontitis.

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Published

2018-08-06

How to Cite

Aguilar Betancourt, A. J., Tello, G., Zamora Gutierrez, L., & González Eras, S. P. (2018). Molecular diagnosis of microorganisms periodontopathogens in alcoholic - smoking patients with chronic periodontitis of the city of Loja, Ecuador. Odontología, 20(1), 33–49. https://doi.org/10.29166/odontologia.vol20.n1.2018-33-49

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Section

Scientific article

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