Gene expression of BMPR1, BMPR2, and TGFBR1 in human dentin treated with different demineralizing solutions and adhesion of cultured apical papilla cells

Abstract

The success of regenerative endodontic procedures depends on the decontamination of the endodontic space, the presence of undifferentiated mesenchymal cells, the release of growth factors that act as signaling molecules for cell attraction, proliferation and differentiation; and the presence of a “scaffold” that supports the organization and vascularization of the newly formed tissue. Objetive. Analyze specific chelating solutions of calcium EGTA, Citric Acid and EDTA in the release of growth factors (BMPR1, BMPR2 and TGFBR1) present in dentin as cell adhesion. Materials and Methods. Dentin discs were obtained from human premolars and third molars, after immersion for 1 minute with EDTA, EGTA and Citric Acid, the cells were seeded. Cell adhesion was assessed at 48 hours using MEV. Gene expression was determined at 7 and 21 days of cell culture. The statistical analysis was ANOVA followed by Tukey, with a significance level of 5%. Results. At 21 days, EGTA treatment significantly increased BMPR1 compared to PBS. No experimental conditions altered the expression of BMPR2. TGBR1 expression at 21 days increased significantly to EGTA treatment. Although there was not a uniform distribution of cells throughout the discs, cell adhesion was observed in all experimental groups. Conclusions. The type of demineralizing solution interferes with the amount of growth factors BMPR1 and TGFBR1 released from human dentin and does not interfere with cell adhesion.