Molecular identification of Brucella abortus isolated from lymph nodes of cattle slaughtered in Loja
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Abstract
Brucellosis is an infectious disease caused by microorganisms of the genus Brucella that affect different species of mammals. The bacteria cause abortion, epididymitis, vesiculitis, birth of weak calves, the decrease in milk production, and infertility in cows and bulls. The disease is a zoonosis and is considered a public health problem. The objective of this work was to identify Brucella abortus by the Polymerase Chain Reaction. It was possible through the amplification of a fragment of 223 bp of the BCSP31 gene region, as DNA source, obtained from lymph nodes of the region cervical, pulmonary, bronchial, retropharyngeal, and mediastinal. One hundred and fifteen samples from animals slaughtered at Loja city abattoir in 2015 were analyzed. In 14.8% (17) of the slaughtered animals, the test was positive; although the presence of the bacteria was observed more frequently in the cervical, bronchial and retropharyngeal regions. The age of the positive animals ranged between 4 and 6 years. With the present study, it was confirmed the presence of the bacterium Brucella abortus through the molecular PCR test obtained from lymph nodes of slaughtered animals in the Loja canton.
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