Physiological quality of Vasconcellea pubescens A. DC seeds as a function of moisture content and pre-germination treatments
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Abstract
Understanding seed physiological quality and germination control significantly contribute to use and conservation of native species. Vasconcellea pubescens A. DC. is important due to its nutritional value in Peru; however, one of the main limitations of this species is seed dormancy. The objective of this study was to evaluate the influence of seed moisture content and seven pre-germination treatments on the physiological quality of seeds stored for six years at 10 °C and 50% relative humidity.
The experiment was conducted under a completely randomized design with a factorial arrangement with two seed moisture contents (8 and 9%) and seven pre-germination treatments (GA₃ 200 ppm for 24 h, GA₃ 400 ppm for 24 h, KNO₃ 2.5% for 24 h, KNO₃ 1.5% for 24 h-1, mechanical scarification, hot water at 70 °C for 5 min, and a control). Analysis of variance (ANOVA) was performed at a 5% significance level using Tukey’s mean comparison test (α = 0.05).
The results showed there was a significant effect of ¨moisture content” and “pre-germination treatments” on germination. Mechanical scarification and GA₃ at 200 ppm promoted the highest germination (up to 80%). In contrast, KNO₃ and hot water treatments resulted in high percentages of non-germinated seeds, while the application of GA₃ at 400 ppm caused cotyledon oxidation.
In this context, storing V. pubescens seeds at 9% moisture content keeps their viability and enhances their response to pre-germination treatments, representing an effective alternative for ex situ conservation and ensuring the preservation and sustainable use of this species.
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