Evaluation of the microhardness of enamel affected by incipient caries and treated by two types of fluoride varnishes: In vitro study
DOI:
https://doi.org/10.29166/odontología.vol19.n2.40-52Keywords:
Permanent dentition, dental caries, fluorine, dental enamelAbstract
Fluoride has shown its effectiveness in the therapy of initial lesions of caries. Currently, there are new fluoride varnishes with additional remineralizing agents, to treat lesions of non-cavitated caries in a non-invasive way, promoting the remineralization of the enamel. Objective: To determine the microhardness of enamel with incipient caries lesions treated with two fluoride varnishes. Materials and methods: In vitro experimental study. The sample consisted of 35 enamel blocks, which were randomly divided into 3 groups. G1 (n=5): negative control; G2 (n=15): Fluorine varnish (Flúor protector - Ivoclar Vivadent) and G3 (n=15): Fluorine varnish with Tricalcium Phosphate (Clinpro White Varnish - 3M ESPE). Three indentations were made by means of a microhardness meter, in order to obtain the initial hardness, then the samples were demineralized with the purpose of producing incipient lesions, where 3 indentations were made again to obtain the microhardness of the demineralized enamel, after this phase the remineralizing solution in each sample and a cyclical pH regime was followed during 7 days to simulate the oral conditions, after this period 3 indentations were made in each sample to obtain the final hardness of the remineralized enamel, reaching a total of 9 indentations per sample. The data were analyzed using the Scheffé test and the Student’s T test with a level of significance of 5%. Results: There was a significant difference between the fluoride varnish with Tricalcium Phosphate (TCP) and the control group (p=0.03). No significant differences were found between the different fluorinated varnishes (p=0.09). A significant improvement of the microhardness after treatment of both fluoride varnishes was observed (p<0.001). Conclusion: The two types of fluoride varnishes efficiently increased the microhardness of enamel with incipient caries lesion, without significant difference between these two.
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