Histomorfometric Study of the bone regeneration using bifasic calcium sulfate combined with alendronate in rabbits
Keywords:
Bone regeneration, Femur, Calcium sulfate, Alendronate, BisphosphonateAbstract
Alendronate is a bisphosphonate with a broad spectrum of indications whose main capacity is the inhibition of osteoclastic function. Biphasic calcium sulfate is an alloplastic graft that possesses the advantages of simple calcium sulfate as biocompatibility, osteoconductive and bioreabsorption properties. Objective: To determine the efficacy of bone regeneration through a histomorphometric study using biphasic calcium sulfate alone and combined with Alendronate. Materials and methods: 24 femurs of Andean male rabbits were used between 1.5 and 2.5 Kg, divided into 3 groups: G1 Physiological bone regeneration, G2 Biphasic calcium sulphate and G3 Biphasic calcium sulphate combined with alendronate. An incision of 2.5 cm was made to create defects of 5 mm in diameter with a depth of 1.5 mm. All the animals were sacrificed at the sixth week and histomorphometriccuts were obtained. The statistical tests of ANOVA and Tukey with a level of significance of 5% were used. Results: Bone regeneration was observed in 10.63%, 40% and 71.88% for G1, G2 and G3 respectively. A statistically significant difference was found between the groups (p <0.001). A difference was observed between G1 and G3 (p <0.001), as well as between G2 and G3 (p = 0.05), being the best results found for the G3.Conclusion: Biphasic calcium sulphate combined with Alendronate showed better bone regeneration when compared to physiological regeneration and calcium sulfate groups in rabbits.
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