In vitro propagation of two promising genotypes of Plukenetia volubilis L.
Article Sidebar
Main Article Content
Abstract
This study was conducted to determine the optimal balance of the auxins 1-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) for the induction of adventitious roots in nodal segments of sacha inchi (Plukenetia volubilis L.). Zygotic embryos from two promising sacha inchi genotypes, “Pinto Recodo” and “Mushquiyacu,” were cultured on a germination medium. Subsequently, after 90 days of in vitro culture, the resulting plantlets were sectioned to obtain nodal segments containing one to two axillary buds, which were then transferred to rooting media supplemented with the growth regulators NAA and IBA.
The highest percentage of adventitious root induction was achieved when IBA alone at 1 mg L⁻¹ was used. The greatest root length was recorded when IBA at 1.0 and 2.0 mg L⁻¹ was applied in the medium for the “Mushquiyacu” genotype, whereas in the “Pinto Recodo” genotype, this response was observed only with 1.0 mg L⁻¹ IBA. The combination of NAA and IBA was not effective in either sacha inchi genotype.
The use of the commercial substrate Premix during plantlet acclimatization proved to be suitable for the survival of both promising sacha inchi genotypes during the nursery stage and after transplantation to the field.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The authors who publish in Siembra know and accept the following conditions:
- Authors retain the copyright and grant Siembra the right of first publication of the work, under the Creative Commons Attribution License. Third parties are allowed to use what has been published as long as they refer to the author or authors of the work and its publication in this journal.
This content is licensed under a Creative Commons Attribution-Noncommercial 4.0 International (CC BY-NC 4.0).
- Authors maintain the copyright and guarantee Siembra the right to publish the manuscript through the channels it considers appropriate.
- Authors may establish on their own additional agreements for the non-exclusive distribution of the version of the work published in Siembra, acknowledging their initial publication in the same, such as in institutional repositories.
- Authors are authorized to disseminate their work electronically once the manuscript is accepted for publication.
References
Agbo, E.A., Ugese, F.U., y Baiyeri, P.K. (2015). Vegetative propagation of African walnut (Plukenetia conophora) using pre-treated stem cuttings of varied physiological ages. Agro-Science, 13(1), 37-43.https://doi.org/10.4314/as.v13i1.6
Amadi, J.O., Appah, O.R., Ibode, T.R., y Agbonavbare, O.R. (2018). Effect of temperature and duration of storage on viability of Plukenetia Conophora (Walnut) mull arg seeds using tetrazolium test. International Journal of Research Agricultural Science, 5(2), 104-107. https://www.ijras.org/index.php/archive?view=publication&task=show&id=296
Bordignon, S. R., Ambrosano, G. M. B., y Rodrigues, P. H. V. (2012). Propagação in vitro de sacha inchi. Ciência Rural, 42(7), 1168–1172. https://doi.org/10.1590/S0103-84782012005000049
Bussmann, R. W., Téllez, C., y Glenn, A. (2009). Plukenetia huayllabambana sp. nov. (Euphorbiaceae) from the upper Amazon of Peru. Nordic Journal of Botany, 27(4), 313–315. https://doi.org/10.1111/j.1756-1051.2009.00460.x
Díaz, L. P., Medina, L. F., Latife, J., Digonzelli, P. A., y Sosa, S. B. (2004). Aclimatación de plantas micropropagadas de caña de azúcar utilizando el humus de lombriz. RIA. Revista de Investigaciones Agropecuarias, 33(2), 115-128. https://www.redalyc.org/articulo.oa?id=86433208
Feng, C., Zhu, Y., Su, Q., Zhou, X., Chen, W., Tao, Y.-B., Chen, M., He, H., Pan, B.-Z., Xu, Z.-F., y Fu, Q. (2025). Reproductive biology of Plukenetia corniculata Sm. (Euphorbiaceae), a traditional wild vegetable in Southeast Asia. Genetic Resources and Crop Evolution, 72(1), 107–119. https://doi.org/10.1007/s10722-024-01967-8
Gillespie, L. J. (1993). A synopsis of neotropical Plukenetia (Euphorbiaceae) including two new species. Systematic Botany, 18(1),575–92. https://doi.org/10.2307/2419535
Henao Ramírez, A. M., Urrea Trujillo, A. I., y Atehortúa Garcés, L. (2021). In vitro germination and vegetative propagation through bud development of sacha inchi (Plukenetia volubilis L.). Acta Biológica Colombiana, 27(1), 70-78. https://doi.org/10.15446/abc.v27n1.88727
Istiandari, P., y Faizal, A. (2025). Integrating in vitro cultivation and sustainable field practices of sacha inchi (Plukenetia volubilis L.) for enhanced oil yield and quality: A Review. Horticulturae, 11(2), 194. https://doi.org/10.3390/horticulturae11020194
Kim, D.-S., y Joo, N. (2019). Nutritional composition of sacha inchi (Plukenetia Volubilis L.) as affected by different cooking methods. International Journal of Food Properties, 22(1), 1235–1241. https://doi.org/10.1080/10942912.2019.1640247
Kodahl, N., García-Dávila, C. R., Cachique, D., Sørensen, M., y Lütken, H. (2018). An in vitro seed germination protocol for Plukenetia volubilis L. Acta Horticulturae, (1201), 549–554. https://doi.org/10.17660/ActaHortic.2018.1201.73
Lee, J.-W., Do, G.-R., Jo, I.-H., Hong, C.-E., Bang, K.-H., Kim, J.-U., y Park, Y.-D. (2021). Zygotic embryo culture is an efficient way to optimize in vitro growth in Panax ginseng. Industrial Crops and Products, 167, 113497. https://doi.org/10.1016/j.indcrop.2021.113497
Mállap-Detquizán, G., Vilca-Valqui, N. C., Meléndez-Mori, J. B., Huaman-Huaman, E., y Oliva, M. (2021). Multiplicación in vitro de pitahaya amarilla (Hylocereus megalanthus) a partir de plántulas obtenidas in vitro. Agronomía Mesoamericana, 45472. https://doi.org/10.15517/am.v33i1.45472
Millones, C.E., y Vásquez, E. R. (2008). Micropropagación de plantas de sacha inchi (Plukenetia volubilis L.) provenientes de la provincia de Rodríguez de Mendoza, región Amazonas. Investigaciones Amazonenses, 2(1), 7-11.
Millones, C. E., y Vásquez, E. R. (2010). Micropropagación de plantas derivadas de semillas botánicas de pitahaya amarilla (Selenicereus megalanthus Britton & Rose) provenientes de la provincia de Utcubamba, región Amazonas. Investigaciones Amazonenses, 4(1), 34-38.
Millones, C.E. y Vásquez, E. R. (2020). Regeneración y enraizamiento de brotes adventicios etiolados de cultivares de zarzamora (Rubus sp.). Revista de Investigaciones Altoandinas, 22(4), 330-342. https://doi.org/10.18271/ria.2020.195
Niazian, M., y Niedbała, G. (2020). Machine learning for plant breeding and Biotechnology. Agriculture, 10(10), 436. https://doi.org/10.3390/agriculture10100436
Murashige, T., y Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Restrepo-Osorio, C., Gil-Correal, A., Chamorro-Gutiérrez, L., Ramírez-Ríos, V., Álvarez, J. C., y Villanueva-Mejía, D. (2020). Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.). BMC Research Notes, 13(1), 414. https://doi.org/10.1186/s13104-020-05257-1
Ruiz-Solsol, H., y Mesén, F. (2010). Efecto del ácido indolbutírico y tipo de estaquilla en el enraizamiento de sacha inchi (Plukenetia volubilis L.). Agronomía Costarricense, 34(2), 259-267. https://doi.org/10.15517/rac.v34i2.3636
Solis, R., Cachique, D., Guerrero-Abad, J. C., Ruiz, M. E. y Tapia, L. (2018). In vitro propagation of sacha inchi through organoigenesis. Pesquisa Agropecuária Brasileira, 53(11), 1285-1288. https://doi.org/10.1590/s0100-204x2018001100012
Solis Leyva, R., Gonzales Chanzapa, N., Pezo Najar, J. M., Arévalo López, L. A., y Vallejos Torres, G. (2019). Rooting of sacha inchi (Plukenetia volubilis) juvenile cuttings in microtunnels. Acta Agronómica, 68(1), 35-40. https://doi.org/10.15446/acag.v68n1.72101
Valente, M. S. F., Lopes, M. T. G., Chaves, F. C. M., Pantoja, M. C., Sousa, F. M. G., y Chagas, E. A. (2017). Molecular genetic diversity and mating system in sacha inchi progenies. Pesquisa Agropecuária Tropical, 47(4), 480–487. https://doi.org/10.1590/1983-40632017v4749799
Valles, C. R. (1990). El "sacha inchi", planta nativa de importancia proteica y aceitera promisora para la selva alta. UNSM, Tarapoto. Separata.
Wang, S., Zhu, F., y Kakuda, Y. (2018). Sacha inchi (Plukenetia volubilis L.): Nutritional composition, biological activity, and uses. Food Chemistry, 265, 316–328. https://doi.org/10.1016/j.foodchem.2018.05.055